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KMID : 1024420130170020165
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Food Engineering Progress
2013 Volume.17 No. 2 p.165 ~ p.171
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Purification of β-Mannanase from Xylogone sphaerospora and the Effect of Picea abies Galactosyl Glucomannan Hydrolysates on the Growth of Bifidobacterium spp.
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Lee Myung-Seok
Park Young-Seo
Park Gwi-Gun
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Abstract
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β-Mannanase from Xylogone sphaerospora was purified by Sephadex G-100 column chromatography. The specific activity of the purified enzyme was 8.24 units/mL protein, representing an 58.86-fold purification of the original crude extract. The final preparation thus obtained showed a single band on SDS-polyacrylamide gel electrophoresis. The molecular weight was determined to be 42 kDa. Picea abies galactosyl glucomannan was hydrolyzed by the purified β-mannanase, and then the hydrolysates were separated by activated carbon column chromatography. The main hydrolysates were composed of D.P. (degree of polymerization) 7, 8, 12, and 13 galactosyl glucomanno-oligosaccharides. To investigate the effects of Picea abies galactosyl glucomanno-oligosaccharides on the in vitro growth of Bifidobacterium longum, B. bifidum, B. animalis, B. breve, B. infantis, B. adolescentis, and B. auglutum, Bifidobacterium spp. were cultivated individually on a modified-MRS medium containing a carbon source such as D.P. 7, 8, 12, and 13 galactosyl glucomanno-oligosaccharides. B. longum propagated 10.83-fold, 12.50-fold, 10.25-fold, and 9.25-fold more effectively by the treatment of D.P. 7, 8, 12, and 13 galactosyl glucomanno-oligosaccharides, respectively, compared to those of standard MRS medium. Especially, all four sorts of galactosyl glucomannooligosaccharides were more effective in promoting the growth of B. longum than B. animalis, B. bifidum, B. breve, and B. infantis.
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KEYWORD
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galactosyl glucomanno-oligosaccharides, Xylogone sphaerospora, β-mannanase, Bifidobacterium spp.
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